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Column Method

  • Simply P Virus RNA Extraction Kit MORE
    Product Details:

    Main constituents

     

    Cat#

    BSC56S1

    BSC56M1

    BSC56L1

     

    Ingredients

    Kit Content

    50T

    100T

    200T

    Lysis Buffer

    25 ml

     50 ml

    100 ml

    Salt and Tris-HCl Buffer

    Wash Buffer I

    18ml

    36ml

    72ml

    High-salt solution

    Wash Buffer II

    12ml

    24ml

    48ml

    Low-salt solution

    RElution Buffer

    10ml

     20ml

    40ml

    RNase-free H2O

    Spin Columns

    50

     100

    200

    Plastic parts and nucleic acid adsorption film

    Handbook

    1

     1

    1

     

    PSBuy BSC56S add 12ml Absolute ethanol to 18ml Wash Buffer I before use. add 48ml Absolute ethanol to 12ml Wash buffer II before use.

    Buy BSC56M1 add 24ml Absolute ethanol to 36ml Wash Buffer I before use. add 96ml Absolute ethanol to 24ml Wash buffer II before use.

    Buy BSC56L1 add 48ml Absolute ethanol to 72ml Wash Buffer I before use. add 192ml Absolute ethanol to 48ml Wash buffer II before use.

    Product Features:

    Reagent prepared by the user

    Absolute ethanol (AR).

     Storage and transportation

    1)  The kit can be transported at room temperature.

    2)  The kit has demonstrated stability of 12 months when stored at room temperature.

    Notice

    1)  Lysis Buffer may be precipitated at low temperature, please heated at 56 ℃ for a few minutes to restore the clarification.

    2)  Wash Buffer I and Wash Buffer II add the absolute ethanol as the volume marked on bottle label and mix well.

    Experimental data:

    Purification coronavirus RNA from cat ascites by this kit, real-time PCR result.

    Manual download
  • Simply P Total RNA Extraction kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC52S1

    BSC52M1

    Components

    50Tests

    100Tests

    Solution R1

    10ml

    20ml

    Solution R2

    30ml

    60ml

    Wash Buffer

    25ml

    (add 75ml ethanol before use)

    25ml×2

    (add 75 ml ethanol before use)

    Elution Buffer

    10ml

    20ml

    Spin columns

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit is a ready-to-use reagent for the isolation of total RNA from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. Add Solution R2 to the processed sample and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis ,construction cDNA library etc.

    Product Features:

    Storage and transportation

    •  The kit has demonstrated stability of 24 months when stored at room temperature.
    •  The kit can be transported at room temperature.

    Technical Information

    Method

    Time

    Max volume

    Yield

    Spin column

    7~15min

    750µl

    ≥90%

    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm                          * Absolute ethanol

    *  Vortex mixer

    Important note

    Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

    Experimental data:

    Analysis RNA

    Absorbance analysis of yield and purity

    lPrepare RNA, dilute by 25mM Tris-HCl(pH7.5), RNase-free water or TE buffer in a right factor;

    lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer;

    lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

    Final concentration = (Spec reading A260) × (Dilution factor)  × (Conversion factor A260)

    ð The conversion factor for RNA is 0.040μg/μl per OD260 unit

    ð Notice: the range of Spec reading A2600.1≤OD260≤1.0

    Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/ (Spec.readingA280)

    ð Ration of 1.8~2.0 are considered ideal purity.

    The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.

    Picture 1:Animal

    Manual download
  • Biospin Whole Blood Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC06S1

    BSC06M1

    Component

    Amount

    Amount

    PK solution

    0.5ml

    1.0 ml

    LysisB Buffer

    10.0ml

    20.0 ml

    WB1 Buffer

    11.825ml

    23.65ml

    Wash Buffer

    11.55ml×2

    23.1ml×2

    Elution Buffer

    10ml

    20ml

    Spin column

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit provide a very simple, fast and effective technique for the isolation of pure high–molecular-weight genomic DNA in whole blood which was treated with anticoagulants such as citrate, heparin,  EDTA. The kit is also suitable for the extraction of genome DNA from leukocytes. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely  avoided.In  general,  2~6μg  genomic  DNA  can  be  acquired  from 200μl blood using the kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

    Product Features:

    Storage

    • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
    • All components, when stored properly, can keep stable for 18 months.

    Apparatus and Materials should Be Supplied by the User

    *   1.5ml sterile Micro-centrifuge tubes        * 10µl/100µl/1000µl tips

    *   Micro-centrifuge capable of 14,000×g    * Absolute ethanol (>99%)

    *   Vortex mixer

    Experimental data:

    Analysis DNA

    •  Absorbance analysis

    Take some DNA,dilute it into an appropriate concentration with elution buffer.

    Measure it at   OD260   ,OD280  and OD320.

    Equation:concentration(μg/ml)=50×OD260×dilution multiple Target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

    •  Agarose Gel Analysis

    0.8~1% Agarose gel

    Example 1:Blood

    Manual download
  • Biospin Yeast Plasmid DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC01S1G

    BSC01M1G

    Components

    50Tests

    100Tests

    RS Buffer

    30.0ml

    60.0ml

    Resuspension Buffer

    12.5ml

    25ml

    Lysis Buffer

    12.5ml

    25ml

    Neutralization Buffer

    17.5ml

    35ml

    PW Buffer

    25ml

    50ml

    Wash Buffer

    13.0ml

    (add 52ml ethanol before use)

    26.0ml

    (add 104ml ethanol before use)

    Snailase

    260mg×2 (2-8℃)

    260mg×4(2-8℃)

    RNase A

    50ul  (2-8℃)

    100ul (2-8℃)

    Elution Buffer

    10.0ml

    20.0ml

    Spin columns

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The Kit provides a fast, simple, and cost-effective yeast plasmid miniprep method for routine  molecular biology laboratory applications. Yeast plasmid DNA can be purified from 3–5ml of overnight cultures of yeast. The DNA isolated by this Kit is ready for downstream applications such as transformation, sequencing, PCR/Real-time PCR and other downstream experiments.

    Product Features:

    Storage and Transportation

    • The kit should be stored at room temperature(15~25℃), but the Snailase and the RNase A should be stored at 2~8℃,the Snailase solution should be stored at -20℃.The kit can be stored for up to 12 months by this method. After addition of RNase A, Resuspension Buffer should be stored 2~8℃.
    • The kit can be transported at room temperature.

    Materials to Be Supplied by the User

    * Sterile 1.5ml micro centrifuge tubes     * Absolute ethanol     *β-mercaptoethanol

    Important notes

    1. Please use 1.3 ml RS Buffer to oscillation dissolve each tube snailase, then hold - 20 ℃.
    2. The β-mercaptoethanol should be added into the RS Buffer (10ul/ml) before use. This mixture is  good at room temperature for 1 week.
    3. The RNase A should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
    4. Add ethanol (as the volume is marked on bottle label) to Wash Buffer then mix well.
    5. If the Lysis Buffer and Neutralization Buffer precipitated, it should be redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
    6. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
    7. The Kit can extract high-quality yeast plasmid DNA from 3-5ml yeast culture overnight cultured.
    Experimental data:

    DNA Analysis

    • Absorbance analysis

    Get some plasmid DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.8

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis
      0.8~1% Agarose gel

    Example 1:Plasmid DNA electrophoresis

    DL15, 000                                    DL2, 000

    Example 2:Enzymatic reactions analysis

         DL2, 000                                                                                                         DL15, 000

    Manual download
  • Biospin Whole Blood Genomic DNA Midi Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC06S1B

    Components

    10Tests

    PK solution

    1.5ml

    Balance Buffer

    10ml

    5×RCL Buffer

    60ml

    NS Buffer

    20ml

    LysisB Buffer

    20ml

    WB1 Buffer

    17.2ml

    (add22.8ml ethanol before use)

    Wash Buffer

    21ml

    (add 49ml ethanol before use)

    Elution Buffer

    20.0ml

    MidiSpin column

    10

    Handbook

    1 copy

    Introduction

        The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood which was treated with anticoagulants such as citrate, heparin, EDTA. Based on the remarkable selectivity on genome DNA of Midispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic   DNA within 2hours. No any expensive equipment are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. In general, 150~250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、 Southern blot、mutant analysis、SNP, and so on.

    Product Features:

    Storage

    • The PK solution and 5×RCL Buffer must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
    •  All components, when stored properly, can keep stable for 18months.

    Apparatus and Materials should Be Supplied by the User

    *  50ml sterile Micro-centrifuge tubes                 * 10µl/100µl/1000µl tips

    *  Micro-centrifuge capable of 5,000×g               * Absolute ethanol (>99%)

    *  Vortex mixer                                                 * warm bath

    Before Starting:

    (1)     According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.

    (2)       Add 4 volumes of deionized water to 1 volume of 5×RCL Buffer.

    (3)      Add 1ml Balance Buffer to each MidiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Take some DNA,dilute it into an appropriate concentration with elution buffer. Measure it at OD260,OD280 and OD320.

    Equation:concentration(μg/ml)=50×OD260×dilution multiple

    target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

    •  Agarose Gel Analysis

    0.8~1% Agarose gel

    Manual download
  • Biospin Whole Blood Genomic DNA Maxi Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC06S1C

    Components

    10Tests

    PK solution

    5ml

    Balance Buffer

    20ml

    LysisB Buffer

    70ml×2

    WB1 Buffer

    25.8ml

    (add 34.2ml ethanol before use)

    Wash Buffer

    64ml

    (add 96ml ethanol before use)

    Elution Buffer

    20.0ml

    MaxiSpin column

    10 tubes

    Handbook

    1 copy

    Introduction

        The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood based on the remarkable selectivity on genome DNA of Maxispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 2hours. No any expensive equipment are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general, 150~250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

    Product Features:

    Storage

    • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
    • All components, when stored properly, can keep stable for 18months.

    Apparatus and Materials should Be Supplied by the User

    *  50ml sterile Micro-centrifuge tubes       * 10µl/100µl/1000µl tips

    *  Micro-centrifuge capable of 5,000×g     * Absolute ethanol (>99%)

     *  Vortex mixer                                      * warm bath

    Before Starting:

    1. According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.
    2. Add 2 ml Balance Buffer to each MaxiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

    Note:Each elution typically yields 60%of DNA bound to the column. To obtain DNA at higher concentrations, please suck the liquid collected in the collection tube again to the column. Incubate at room temperature for 2 minutes. Centrifuge at 4,000×g for 3 minutes. The DNA in the collection tube is ready for further analysis. If the isolated DNA sample is not going to be tested on the same day, freeze it at -20℃.

    Experimental data:

    Analysis DNA

    •  Absorbance analysis

    Take some DNA,dilute it into an appropriate concentration with elution buffer. Measure it at OD260,OD280 and OD320.

    Equation:concentration(μg/ml)=50×OD260×dilution multiple

    target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

    • Agarose Gel Analysis 

    0.8~1% Agarose gel

    Example 12ml whole blood


    Example 25ml Blood clots


    Manual download
  • Biospin Virus RNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC62S1

    BSC62M1

    Components

    50Tests

    100Tests

    RLysis Buffer

    15ml

    30ml

    RD Buffer

    17.5ml

    35ml

    DNase Stop Buffer

    5.0ml

    (add 6.5ml ethanol before use)

    10.0ml

    (add 13ml ethanol before use)

    Wash Buffer

    20ml

    (add 30ml ethanol before use)

    40ml

    (add 60ml ethanol before use)

    RElution Buffer

    10ml

    20ml

    Spin Columns

    50

    100

    Handbook

    1copy

     1copy

    Introduction

        The kit is a ready-to-use reagent for the isolation of Virus RNA from different type’s sample. Add RLysis Buffer to the processed sample and adding alcohol will bind RNA to spin column. Then RNA can be easily isolated through several washing and eluting steps.

        The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation, RNase protect assay, RT-PCR/Real-time RT-PCR analysis, construction cDNA library etc.

    Product Features:

    Storage and transportation

    • The kit has demonstrated stability of 24 months when stored at room temperature.
    • The kit can be transported at room temperature.

    Technical Information

    Sample

    Amount

    Animal tissue

    ≤30mg

    Culture cells

    ≤1×108

    White blood cells

    ≤from 5ml whole blood

    For liquid sample

    ≤100μl

    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml microcentrifuge tubes            * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm       * 70℃ Water bath      * Vortex mixer

    *  Liquid nitrogen (or ice bath)     * Optional: β-Me        * PBS          *Trypsination

    *  Red Blood Cells Lysis Buffer(Cat#BSA06M1)         * Absolute alcohol

    Important note

    • RD Buffer may be precipitated at low temperature, the heated at 37 ℃ for a few minutes, to restore the clarification.
    • DNase Stop Buffer Add the alcohol as the volume marked on bottle label and mix well.
    • Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.
    Experimental data:

    Analysis RNA

    ExampleHCV Real-time RT-PCR  (Sample HCV concentration:copies/ml)

    Manual download
  • Biospin Total RNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC63S1

    BSC63M1

    Components

    50Tests

    100Tests

    RLysis Buffer

    8.75ml

    17.5ml

    RD Buffer

    17.5ml

    35ml

    DNase Buffer

    2ml

    4ml

    MnCl2

    450ul

    900ul

    DNase I

    50ul(stored at -20℃)

    100ul(stored at -20℃)

    DNase Stop Buffer

    5 ml

    (add 6.5 ml ethanol before use)

    10 ml

    (add 13 ml ethanol before use)

    Wash Buffer

    32 ml

    (add 48ml ethanol before use)

    32 ml

    (add 48ml ethanol before use)×2

    RElution Buffer

    10ml

    20ml

    Spin columns

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit is a ready-to-use reagent for the isolation of total RNA from animal tissues, cells, bacteria and others (plant tissues are not recommended). Add RLysis Buffer to the processed sample.RD buffer will remove the protein, then adding alcohol will bind RNA to spin column. The DNA will be destroyed by the DNase I reaction. Then RNA can be easily isolated through several washing and eluting steps.

        The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation,RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library etc.

    Product Features:

    Storage and transportation

    • The kit has demonstrated stability of 18 months when the DNase I should be stored at -20 ℃, others at room temperature.
    • The kit can be transported at room temperature.

    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml centrifuge tubes                       * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm           * Absolute alcohol

    *  Vortex mixer                                              * β- mercaptoethanol

     Important note

    • DNase Stop Buffer: Add the alcohol as the volume marked on bottle label and mix well.
    • Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.
    Experimental data:

    Analysis RNA

    • Absorbance analysis

    Get some RNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=40×OD260×dilution fact

    Target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis

    Manual download
  • Biospin SwabGen DNA Extraction Kit MORE
    Product Details:

     Kit Components

     

    Cat#

    BSC28S1

    BSC28M1

    Components

    50Tests

    100Tests

    PK solution

    500ul

    1000ml

    LysisB Buffer

    30ml

    60ml

    WB1 Buffer

    12ml

    (add 17ml ethanol before use)

    24ml

    (add 34ml ethanol before use)

    Wash Buffer

    18ml

    (add 42ml ethanol before use)

    36ml

    (add 84ml ethanol before use)

    Elution Buffer

    10 ml

    20 ml

    Spin column

    50 tubes

    100 tubes

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in Oral swabs. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general,   0.5~3.5μg genomic DNA can be acquired using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

    Product Features:

    Storage and transportation

    • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
    • All components, when stored properly, can keep stable for 18months.
    • The kit can be transported at room temperature.

    Apparatus and materials to be prepared by the user

    *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

    *  Micro-centrifuge capable of 12,000×g                * Absolute ethanol (>99%)

    *  Vortex mixer                                                  * Warm bath or metal bath

    Important note

    Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

    Experimental data:

    Analysis DNA

    •  Absorbance analysis

     Take some DNA,dilute it into an appropriate concentration with elution buffer.

     Measure it at OD260,OD280 and OD320.

    Equation:concentration(μg/ml)=50×OD260×dilution multiple

    target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

    • Agarose Gel Analysis 0.8~1% Agarose gel

     SYBR Green Real-time PCR

    Manual download
  • Biospin Polyacrylamide Gel DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC15S1

    BSC15M1

    Components

    50Tests

    100Tests

    PE Buffer

    10 ml

    20 ml

    PEB Buffer

    20 ml

    40 ml

    PWash Buffer

    18.9ml

    37.8ml

    Elution Buffer

    10ml

    20ml

    Spin Column

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a simple, rapid and effective method for purification of DNA fragments from polyacrylamide gel. The simple purification procedure is based on the remarkable selectivity of Biospin membrane. There need few steps to finish extraction without expensive equipment, as completely avoids using toxic and hazardous reagents such as phenol and chloroform. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, PCR, restriction enzyme digestion, and so on.

    Product Features:

    Storage and transportation

    • The kit should be stored dry at room temperature(15~25℃), The kit can be stored for up to 18 months if all components are kept properly.
    • The kit can be transported at room temperature.

    Technical Information

     Method

    Column volume

    DNA size range

    Electrophoresis buffer

    Incubate temperature

    Elution recovery

    Spin column

         750µl

    100bp~10kb

    TAE/TBE buffer

    65℃

    ≥99%

    Apparatus and Materials to Be Supplied by the User

    * Sterile 1.5 or 2.0 ml microcentrifuge tubes        * 10µl/100µl/1000µl tips

    *  Absolute ethanol    * Microcentrifuge capable of 14,000g * Vortex mixer   * Water bath or cooling & heating block

    Preparation

     

    1. This product cannot be used with PAGE with urea.
    2. Add ethanol as per the volume marked on label of bottle into Wash Buffer and then mix well.
    3. If the gel slice is very big, please increase the dosage of PE Buffer properly but on more than 200μl, thus to guarantee that the gel slice is immerged completely.
    4. The suitable volume of elution buffer for the kit is 50µl. The dosage of elution buffer can be adjusted according to practical experimental situation.
    5. Please wash the fixed gel by water or 10mM Tris-HCl (pH7.0) for 2 or 3 times if the gel is fixed by acidic reagent such as acetic acid.
  • Biospin Plasmid DNA Maxi Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC01S1C

    BSC01M1C

    Components

    10Tests

    25Tests

    Balance Buffer

    20ml

    50ml

    Resuspension Buffer

    100ml

    250ml

    Lysis Buffer

    100ml

    250ml

    Neutralization Buffer

    120ml

    100ml

    Washing Buffer

    50mlх2

    (add 75ml ethanol before use)

    104mlх2

    (add 156ml ethanol before use)

    Elution Buffer

     60ml

    150ml

    RNase A

    1 tube

    1 tube

    Max Spin column

    10 tubes

    25 tubes

    Handbook

    1 copy

    1 copy

    Introduction

         This kit allows the high efficient binding of DNA to our spin matrix while proteins and  other  impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer. This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 ml of E. coli culture. The maxi column has a plasmid DNA binding capacity of 1 mg.

        The purified plasmid DNA is ready for downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

    Product Features:

    Storage and transportation

    •  The kit has demonstrated stability of 18 months when the RNase A should be stored at 2-8℃,others at room temperature.
    • The kit can be transported at room temperature.
    •  Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

    Apparatus and materials to be prepared by the user

     *  50ml centrifuge tubes                            * 10µl/100µl/1000µl tips

     *  Centrifuge capable of 14,000g               * Absolute alcohol

    Important note

    1)Spin down RNase A vial briefly. Add the RNase A solution to Resuspension Buffer and mix well before use. Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

    2)Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.

    3)Lysis Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

    4)Neutralization Buffer may form precipitates below 10°C, warm up at 37°C to dissolve the precipitates before use.

    5)Carry out all centrifugations at room temperature.

    6) Add 2ml Balance Buffer to each MaxiSpin column, incubate at room temperature for 2 mins,centrifuge at 5,000 x g for 3 min. Discard flow-through. It can improve the yield of DNA significantly.

  • Biospin Plasmid DNA Extraction Kit MORE
    Product Details:

    Kit Components 

    Cat#

     

    BSC01M1

     

    BSC01L1

    Components

    100Tests

    250Tests

    Resuspension Buffer

    25ml

    62.5ml

    Lysis Buffer

    25ml

    62.5ml

    Neutralization Buffer

    35ml

    87.5ml

    Wash Buffer

    30 ml

    (add 45ml absolute ethanol before use) ×2

    75 ml

    (add 112.5ml absolute ethanol before use) ×2

    Elution Buffer

    20ml

    50ml

    RNase solution

    1

    1

    Spin columns

    100

    250

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. Plasmid DNA can be purified from 1–5ml of overnight cultures of E. coli. The DNA isolated by this kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-time PCR and other downstream experiments.

    Product Features:

    Storage

    1.The kit should be stored at room temperature (15~25℃), but the RNase solution should be stored at 2~8℃.The kit can be stored for up to 18 months by this method. After addition of RNase solution, Resuspension Buffer should be stored 2~8℃.

    2.The kit can be transported at room temperature.

    Technical Information

     

    Method

     

    Work time

     

    Column volume

     

    Column yield

     

    Elution recovery

     

    Plasmid DNA length

     

    Culture volume

    Spin column

    25 min for

    24 samples

    750µl

    20µg DNA

    ≥99%

    ≤10kB

    1~5ml high-copy plasmid

    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000 × g            * Absolute ethanol          * Vortex mixer

    Important notes

    1. The RNase solution should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
    2. Add ethanol (as the volume is marked on bottle label) to Wash Buffer and mix well.
    3. If the Lysis Buffer and Neutralization Buffer precipitated, it should be Redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
    4. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
    5. The kit can extract high-quality plasmid DNA from 1-5ml E.coli overnight cultured.
    6. The suitable volume is 50ul for Elution Buffer; user can adjust its volume if necessary.
    Experimental data:

    DNA Analysis

    • Absorbance anlysis,

    Get some plasmid DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target:  2.0≥OD260-320/ OD280-320≥1.8

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis,0.8~1% Agarose gel 

     

    Example 2:Enzymatic reactions analysis

    Manual download
  • Biospin Plasma Circulating DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC30S1

    BSC30M1

    Components

    50Tests

    100Tests

    Solution  Ⅰ

    20ml

    40ml

    Solution  Ⅱ

    10ml

    20ml

    PK solution

    500ul  (Store at 2~8℃)

    1000ul  (Store at 2~8℃)

    LysisB Buffer

    10ml

    20ml

    WB1 Buffer

    12ml

    (add 17ml ethanol before use)

    24ml

    (add 34ml ethanol before use)

    Wash Buffer

    18ml

    (add 42ml ethanol before use)

    36ml

    (add 84ml ethanol before use)

    Elution Buffer

    10 ml

    20 ml

    Spin column

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a very simple, fast and effective technique for the isolation of pure plasma DNA. DNA in the sample is released using PK solution and LysisB Buffer. The released DNA is bound exclusively and specifically to the Biospin membrane in presence of LysisB Buffer and ethanol under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed with  twice washing procedures. The DNA is then eluted from the membrane with the elution Buffer. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. The pure DNA can be applied extensively in PCR/Real-time PCR, and so on.

    Product Features:

    Storage and transportation 

    • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
    •  All components, when stored properly, can keep stable for 18months.
    •  The kit can be transported at room temperature.

    Apparatus and materials to be prepared by the user

    *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

    *  Micro-centrifuge capable of 12,000×g                   * Absolute ethanol (>99%)

    *  Vortex mixer                                                                 * Warm bath or metal bath

    Important note

    Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

  • Biospin PCR Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC03S1

    BSC03M1

    Components

    50Tests

    100Tests

    Binding Buffer

    10ml

    20ml

    Wash Buffer

    15ml

    30ml

    Elution Buffer

    10ml

    20ml

    Spin column

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a simple, rapid and effective method for purification of DNA fragments from PCR or enzymatic reaction. DNA fragments ranging from 60bp to 10kb can be purified. The yield of DNA with size lower than 100bp is 23~95%, while the yield of DNA with size from 0.1kb to 10kb is 90~97%. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion, PCR/real-time PCR and so on.

    Product Features:

    Storage and Transportation

    • The kit should be stored dry at room temperature (15~25℃), The kit can be stored for up to 18 months if all components are kept in the manner above.
    • The kit can be transported at room temperature.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5 microcentrifuge tubes          * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g     * Vortex mixer     * Absolute ethanol

    Important notes

    1. Add ethanol (as the volume be marked on bottle label) to Wash Buffer and mix well
    2. Close the lid after using the Binding Buffer as soon as possible.
    3. The suitable volume is 50ul for Elution Buffer; user can adjust its volume if necessary.
    Experimental data:

    Analysis DNA

    • Absorbance anlysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.8

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:

    According to the absorbance and agarose gel analysis,the impurity had been discarded.

    U: unpurified      P: purified               M: Marker

    Example 2:

    Example3:Elution Volume versus DNA Yield

     

    Manual download
  • Biospin Omni Plant Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC13S1B

    Components

    50Tests

    LP Buffer

    22.5 ml

    LP plus Buffer

    22.5 ml

    DA Buffer

    7.5 ml

    P Binding Buffer

    14ml

    G Binding Buffer

    25ml

    Wash Buffer

    25.2ml

    Elution Buffer

    10ml

    Spin column

    50

    Shredder Spin Column

    50

    Handbook

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high– molecular-weight genomic DNA from plant tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and  hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this kit.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

     

    Product Features:

    Storage

    • The kit should be stored at 15-25℃.
    •  All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml microcentrifuge tubes             * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g              * Absolute ethanol            * Vortex mixer

    Important notes

    1. Please add 37.8ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    2. Please add 28ml absolute ethanol to P Binding Buffer and mix thoroughly before the  first use.
    3. LP Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    Absorbance anlysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    expressions:concentration(μg/ml)=50×OD260×dilution fact

    target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:Rice leaves

    Example2: The extraction of plant tissues rich in polysaccharides and polyphenols

                      

    Manual download
  • Biospin miRNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC64S1

    BSC64M1

    Components

    50Tests

    100Tests

    BIOZOL Reagent

    35ml

    70ml

    Wash Buffer

    12ml

    (add 48ml ethanol before use)

    24ml

    (add 96ml ethanol before use)

    Reslution Buffer

    15ml

    30ml

    Spin columns

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit is a ready-to-use reagent for the isolation of total RNA,including miRNA and other small RNA molecules, from cultured cells and various animal and human tissues. Add BIOZOL Reagent to the processed sample, after addition of chloroform, RNA will be isolated from DNA and Protein, and adding alcohol will bind RNA all RNA molecules from 18 nucleotides (nt) upwards to spin column. Then RNA  can be easily isolated through several washing and eluting steps.

        The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library, microarray analysis etc.

    Product Features:

    Storage and transportation

    • The kit has demonstrated stability of 12 months when the BIOZOL Reagent should be stored at 2-8  ℃,others at room temperature.
    • The kit can be transported at room temperature.
    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm                  * Absolute alcohol

    *  Vortex mixer                                                      * Chloroform

    Important note

    Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.

    Experimental data:

    Analysis RNA

    • Absorbance analysis

    Get some RNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320.

    Expressions:concentration(μg/ml)=40×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Real-Time PCR Analysis (Rat rno-miR-21)

    Manual download
  • Biospin Marine Animal Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC27S1

    BSC27M1

    Components

    50T

    100T

    FL Buffer

    30ml

    60ml

    PK Solution

    0.5ml

    1ml

    WS Buffer

    5ml

    10ml

    Binding Buffer

    35ml

    70ml

    PW Buffer

    12ml

    (add 18ml ethanol before use)

    24ml

    (add 36ml ethanol before use)

    Wash Buffer

    26ml

    (add 39ml ethanol before use)

    52ml

    (add 78ml ethanol before use)

    Elution Buffer

    10ml

    20ml

    Spin Column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from marine animal tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA without proteins and other contaminants. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:

    Storage

    • The PK solution is to be stored at 2-8℃, others at 15-25℃.
    •  All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    * Sterile 2.0ml microcentrifuge tubes                * 10µl/100µl/1000µl tips

    * Microcentrifuge capable of 14,000g                * Absolute ethanol

    Important notes

    1. Please add3.6ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
    2. Please add 8.4ml absolute ethanol to Wash Binding Buffer and mix thoroughly before the first use.
    3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.8~1% Agarose gel

    Example 1:Fishtail

    Example 2:Shell meat

    Manual download
  • Biospin Insect Genomic DNA Extraction Kit MORE
    Product Details:
    Kit Components

    Cat#

    BSC26S1

    BSC26M1

    Components

    50Tests

    100Tests

    FL Buffer

    30.0ml

    60.0ml

    PK solution

    1ml

    2ml

    Binding Buffer

    35.0ml

    70.0ml

    PW Buffer

    11ml(add 16.5ml ethanol before use)

    22ml(add 33ml ethanol before use)

    Wash Buffer

    25ml(add 37.5ml ethanol before use)

    50ml(add 75ml ethanol before use)

    Elution Buffer

    10.0ml

    20.0ml

    Spin Column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from insect, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps. It can effectively remove all kinds of pigment, chitin, polysaccharide and other impurities. It can also extract multiple samples once.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:

    Storage

    • The PK Solution is to be stored at 2-8℃, others at 15-25℃.
    • All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5/2.0ml microcentrifuge tubes          * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g               * Absolute ethanol         * Chloroform

    Important notes

    1. Please add 33ml absolute ethanol to PW Buffer and mix thoroughly before the first use.

    2. Please add 75ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.

    3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.

     

  • Biospin Fungus Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components 

    Cat#

    BSC14S1

    BSC14M1

    Components

    50Tests

    100Tests

    LE Buffer

    20 ml

    40 ml

    DA Buffer

    7.5 ml

    15 ml

    E Binding Buffer

    14ml

    (add 28 ml absolute ethanol before use)

    28ml

    (add 56 ml absolute ethanol before use)

    G Binding Buffer

    25ml

    50ml

    Wash Buffer

    25.2ml

    (add 37.8ml absolute ethanol before use)

    50.4ml

    (add 75.6ml absolute ethanol before use)

    Elution Buffer

    10ml

    20ml

    Spin column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from fungus, adopting Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 2.5-30 μg genomic DNA can be acquired from up to 100 mg tissue or up to 5ml yeast culture by using Biospin Fungus Genomic DNA Extraction Kit.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:

    Storage

    • The kit should be stored at 15-25℃.
    • All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g              * Absolute ethanol     * Vortex mixer

    Important notes

    1. Please add absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    2. Please add absolute ethanol to E Binding Buffer and mix thoroughly before the first use.
    3. LE Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.
    4. Prepare Sorbitol buffer: 18.217 g Sorbitol and 3.7224 g EDTA-2Na, dissolved in 100 ml of pure water.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260   ,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact Target:                2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:

    Manual download
  • Biospin FFPE Tissue RNA Extraction Kit MORE
    Product Details:

    Kit Components 

     

    Cat#

     

     

    BSC66S1

     

     

    BSC66M1

    Components

    50Tests

    100Tests

    Deparaffinization Solution

    50ml

    100ml

    Lysis II Buffer

    10ml

    20ml

    Binding Buffer

    10ml

    20ml

    SW Buffer

    26ml

    52ml

    Wash Buffer II

    8ml

    (Add 32 ml ethanol before use)

    16ml

    (Add64 ml ethanol before use)

    RElution Buffer

    10ml

    20ml

    PK Solution

    500µl (Stored at 2-8℃)

    1ml (Stored at 2-8℃)

    Spin Column

    50

    100

    Handbook

    1 copy

    1 copy

    Cat#

    BSA35S2B (Stored at -20)

    BSA35M2B (Stored at -20)

    DNase I Buffer

    1.3 mlх2

    1.3 mlх4

    DNase I

    100µl

    200µl

    Introduction

        This kit is used to extract high- purity RNA from FFPE tissue sections, with non-toxic deparaffinization solution, high-performance Lysis  II Buffer to release RNA from FFPE  efficiently. The high efficient binding of RNA to our spin matrix while proteins and other impurities are removed by   wash buffer. Nucleic acids are easily eluted with sterile RNase free water or RElution Buffer. The purified RNA is ready for downstream applications such as Real-Time PCR,Sec

    Product Features:

    Storage and transportation

    • The kit has demonstrated stability of 12 months when the PK Solution should be stored at 2-8℃,DNase I should be stored at -20℃,others at room temperature.
    • The kit can be transported at room temperature, but PK Solution and DNase I need cold chain.

    Apparatus and materials to be prepared by the user

    *  1.5ml microcentrifuge tubes                        * 10µl/100µl/1000µl tips

    *  Centrifuge capable of ≥14,000g                 * Ethanol(≥95%)

    *  Heating block or water bath

    Important note

    1)Wash Buffer II: Add the ethanol as the volume marked on bottle label and mix well.

    2)Lysis II Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

    3)PK Solution should be stored at 2°C-8℃.

    4)DNase I should be stored at -20°C.

    5)Carry out all centrifugations at room temperature.

    Experimental data:

    Analysis RNA

    Real-Time PCR compare

    Manual download
12
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