Reagent Center

Fast Lysis Method

  • BioFast BIOZOL Total RNA Extraction Reagent MORE
    Product Details:

    Kit Components

    Cat#

    BSC59M1

    Components

    100Tests

     BIOZOL Total RNA Extraction Reagent

    100 ml

     Handbook

     1 copy

     RNA Grind tube

     100 tubes

    Introduction

        BioFast Biozol Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. The operation of the kit is very simple and easy. First, add the sample to the grind tube with Biozol for complete lysis. Second, add chloroform to the solution and then centrifuge the grind tube. The homogenate will be separated to three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

        Since the operation of BioFast Biozol is very easy, it can realize the extraction  for several samples simultaneously. And total RNA isolated by BioFast BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

    Product Features:
    Storage and transportation
    •  1. Recommended to store at 2-8 ℃ and the quality guarantee period is up to one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the performance of the reagent.
    • 2. The kit can be transported at room temperature.
    Important Notes

        RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

     

    • ◆Always wear gloves and respirator in order to prevent RNA degradation.
    • ◆Use sterile, disposable plastic ware and automatic pipettes.
    • ◆All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6-8 hours, while tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temperature in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.
    • ◆Build a separate RNA laboratory with the only instruments if possible.

     

    Experimental data:
    • Absorbance analysis of yield and purity

    lPrepare RNA, dilute by 25mM Tris-HCl(pH7.5), RNase-free water or TE buffer in a right factor;

    lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer;

    lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

    Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)

    • The conversion factor for RNA is 0.040μg/μl per OD260 unit
    • Notice: the range of Spec reading A2600.1 260<1.0

    lCalculate the purity of RNA as follows: Ratio=(Spec.readingA260)/( Spec.readingA280)

    • Ration of 1.8~2.1 is considered ideal purity.

    (The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.)

    Pictures of Experiment

    Picture 1:Total RNA extracted from animal tissues

    Picture 2 Total RNA extracted from rice leaves

                        

    Manual download
  • BioFast Simply P Total RNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC60S1

    Components

    50Tests

     RNA Grind tube

    50 tubes

    Solution R2

    30ml

    Wash Buffer

    18ml (add 54ml ethanol before use)

     Elution Buffer

    10ml

     Spin columns

    50

    Handbook

     1copy

    Introduction

        Biofast-simplyP is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. Add sample to the grind tube with Solution R2 for homogenization and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.

        The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-through put. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+  selection、in  vitro  translation、RNase  protect  assay,  RT-PCR/Real  time  RT-PCR analysis、construction cDNA library etc.

    Product Features:

    Storage and transportation

    •  1. The kit has demonstrated stability of 24 months when stored at room temperature.
    •  2. The kit can be transported at room temperature.

    Apparatus and materials to be prepared by the user

    * Sterile 1.5ml microcentrifuge tubes             * 10µl/200µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm           * Absolute ethanol        *  Vortex mixer

    Important note

    Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

    Experimental data:

    Analysis RNA

    • Absorbance analysis of yield and purity

    lPrepared RNA will be dilute by 25mM Tris-HCl (pH7.5), RNase-free water or TE buffer in a right factor;

    lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl(pH7.5), RNase-free water or TE buffer;

    lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

    Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)

    1. The conversion factor for RNA is 0.040μg/μl per OD260 unit

    2. Notice: the range of Spec reading A260: 0.1≤OD260≤1.0

    Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/( Spec.readingA280)

    3. Ration of 1.8~2.0 are considered ideal purity.

    The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.

    Picture 1:Animal tissues                                     

               

    Picture 2:Rice leaf

             


    Manual download
  • BioFast Soil Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC21S1

    Components

    50Tests

    SP Buffer

    45ml

    Lysis S Buffer

    5ml

    DA Buffer

    12.5ml

    Binding Buffer

    35ml

    Wash Buffer

    30ml(add 45ml absolute ethanol before use)

    Elution Buffer

    15ml

    Grind Tube

    50tubes

    Spin Column

    50tubes

    Handbook

    1copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

        At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

        Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

    Product Features:
    Storage

    All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g              * Absolute ethanol

    Important notes

    • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis

    0.8~1% Agarose gel

    Real-time Taqman PCR detect


    Manual download
  • BioFastSpin Plant Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC18S1

    Components

    50Tests

    LP Buffer

    45 ml

    DA Buffer

    10 ml

    P Binding Buffer

    16ml

    G Binding Buffer

    25ml

    Wash Buffer

    25ml

    Elution Buffer

    10ml

    Spin Column

    50

    Grind Tube

    50

    Handbook

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from plant tissues, Designed for use with the lysis instrument from BIOER,plants are easily lysed within 40 s.The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this Kit.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:
    Storage
    • 1. The kit should be stored at 15-25℃.
    • 2.  All reagents, when stored properly, are stable for 18 months.
    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g              * Absolute ethanol            * Vortex mixer

    Important notes
    • 1. Please add 37.5ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    • 2. Please add 32ml absolute ethanol to P Binding Buffer and mix thoroughly before the first use.
    • 3. LP Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with Elution Buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target:   2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis

    0.8~1% Agarose gel

    Example 1:Rice leaves

    Example 2:Rice leaves


    Manual download
  • BioFastSpin Tissue Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC20S1

    Components

    50Tests

    FL Buffer

    30ml

    Binding Buffer

    35ml

    PW Buffer

    10.5ml

    Wash Buffer

    25.2ml

    Elution Buffer

    10ml

    PK Solution

    0.5ml

    WS buffer

    5ml

    Grind Tube

    50 tubes

    Spin Column

    50 tubes

    Handbook

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than1.5 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 30 μg genomic DNA can be acquired from up to 50 mg tissue by using this kit.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:
    Storage
    • 1. The Protease K is to be stored at 2-8℃, others at 15-25℃.
    • 2. All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 2.0ml microcentrifuge tubes                    * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g                 * Absolute ethanol

    Important notes

    • 1. Please add 15.75ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
    • 2. Please add 37.8ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.
    • 3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37℃ until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target:    2.1≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis

    0.8~1% Agarose gel

    Example 1:Mouse heart and mouse muscle

    B: Bioer Kit

    O: Other company Kit

    Example 2:Mouse liver


     

    Manual download
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Tel:0571-87774567

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