Reagent Center

Extraction Method

  • BIOZOL Total RNA Extraction Reagent MORE
    Product Details:

     

    Cat#

     

    BSC51S1

     

    BSC51M1

    BIOZOL-total RNA Extraction reagent

    30 ml

    100 ml

    Handbook

    1copy

    1copy

    General information

        BIOZOL Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue or from bacterium. The operation of the kit is very simple and easy. First, add the sample into BIOZOL for complete lyses. Second, add chloroform into the solution and then centrifuge the sample tube. Then the homogenate will separate into form three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

        Since the operation of BIOZOL is very easy, it can realize the extraction for several samples simultaneously. And total RNA isolated by BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

    RNA Extraction Procedure

     


    Product Features:

    Storage and Transportation

    ◆   Recommended to store at 2-8℃ and the stability is one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the effect.

    ◆   It can be shipped at room temperature which does not affect its stability.

    Important Notes

        RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

    ◆    Always wear gloves and respirator in order to prevent RNA degradation.

    ◆    Use sterile, disposable plastic ware and automatic pipettes.

    ◆    All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6-8 hours, while  tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temp in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.

    ◆    Build a separate RNA laboratory with the only instruments if possible.

    Experimental data:

     

    Total RNA pictures above are strictly operated according to Biozol extraction protocol.


    Manual download
用戶隱私條款

尊重用戶個人隱私是杭州博日用戶服務的一項基本政策。杭州博日不會在未經合法用戶授權時向第三方公開或透露其注冊資料及保存在杭州博日平臺中的非公開內容,但以下情況除外: ⑴用戶對自身信息保密不當原因,導致用戶非公開信息泄露; ⑵由于網絡線路、黑客攻擊、計算機病毒、政府管制等原因造成的資料泄露、丟失、被盜用或被篡改等; ⑶有關法律要求杭州博日官網服務管理要求提供用戶的個人信息; ⑷在緊急情況下為維護用戶和公眾的生命、財產安全。

免責條款

杭州博日科技不保證本網站上任何內容的準確性、完整性和可靠性以及使用這些內容可能得出的任何結果。?杭州博日科技及相關人不能保證您任何時候均可進入、瀏覽和使用本網站;杭州博日科技對本網站和其內容的不能使用和使用錯誤不承擔任何責任。?本網站對其他網站的鏈接本身并不表示杭州博日科技認可或承擔其他網站內容或使用上的責任。杭州博日科技以及相關人對于在進入、瀏覽和使用本網站、從本網站下載任何內容或通過本網站的任何鏈接進入其他網站而導致病毒或其他破壞性程序對您的電腦系統以及其他任何軟件、硬件、IT系統或財產的損害或損失不承擔任何責任。?杭州博日科技以及相關人對于第三人采用非法手段進入本網站取得有關密碼、資料和內容而引起的任何直接及間接損害或損失不承擔任何責任。最終解釋權歸杭州博日科技所有。

杭州博日保留隨時更正、修改、更新本聲明的權利。

使用條款

本網站由杭州博日科技有限公司(以下簡稱“杭州博日”)創設,本網站提供的任何內容(包括但不限于文字、數據、圖表、圖象、聲音或視頻等)的版權均屬于杭州博日或相關權利人。未經杭州博日或相關權利人事先的書面許可,您不得以任何方式擅自復制、再造、傳播、出版、轉帖、改編或陳列本網站的內容。任何未經授權使用本網站的行為都將違反《中華人民共和國著作權法》和其他相關法律法規以及國際公約的規定,杭州博日充分保留追究相應法律責任的權利。

聯系方式

地址:杭州市高新(濱江)區濱安路1192號(310053)

總機:0571-87774567

网上赚钱项目